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Fig. 9 | Acta Neuropathologica Communications

Fig. 9

From: Anti-α-synuclein immunotherapy reduces α-synuclein propagation in the axon and degeneration in a combined viral vector and transgenic model of synucleinopathy

Fig. 9

Co-localization of neurofilament marker SMI312 and CT-α-syn was reduced in passively immunized α-syn tg mice. To analyze if passive immunization altered the subcellular distribution of CT-α-syn, double labeling of brain sections of α-syn KO, non-tg, and α-syn tg mice that were passively immunized weekly for 13 weeks with either 27-1 (control) or 1H7 (α-syn antibody) beginning one week after LV-α-syn unilateral injection were analyzed using SMI312 antibody against phosphorylated neurofilaments (p-NF) and the CT-α-syn antibody SYN105 was conducted 14 weeks following unilateral LV-α-syn injection. a Representative confocal images of the contralateral neuropil in brain sections from α-syn KO, non-tg, and α-syn tg mice immunized with 27-1 (control), or (b) immunized with 1H7 (antibody). Arrows indicate co-localization of the SMI312 signal with CT-α-syn in neurofilament-like structures. c Analysis of the percentage of co-localization between the neurofilament marker SMI312 and the CT-α-syn antibody SYN105. Error bars represent ± SEM. *P <0.05 when statistically analyzed using a Student’s t-test. Scale bar = 25 μm. α-Syn KO mice + 27-1 (control) n = 8; α-syn KO + 1H7 antibody n = 9; non-tg mice + 27-1 (control) n = 10; non-tg mice + 1H7 antibody n = 9; α-syn tg mice + 27-1 (control) n = 8; α-syn tg mice + 1H7 antibody n = 8

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