Fig. 8

Confocal analysis of phosphorylated neurofilaments in 1H7 passively immunized mice. α-Syn KO, non-tg, and α-syn tg mice were passively immunized weekly for 13 weeks with either 27-1 (control) or 1H7 (α-syn antibody) beginning one week after LV-α-syn unilateral injection. Pathological analysis of axonal density using SMI312 antibody against phosphorylated neurofilaments was conducted 16 weeks following unilateral LV-α-syn injection. a Representative images and (b) computer-aided analysis of axonal density in the contra- and ipsilateral side of α-syn KO mice. c Representative images and (d) computer-aided analysis of axonal density in the contra- and ipsilateral side of non-tg mice. e Representative images and (f) computer-aided analysis of axonal density in the contra- and ipsilateral side of α-syn tg mice. *P-value < 0.05 using ANOVA followed by Dunnett’s post-hoc test comparing 27-1 (control) and 1H7 (antibody) within a hemisphere. ^# P < 0.05 using ANOVA followed by Tukey-Kramer post hoc test comparing contra- and ipsilateral sides. Scale bar = 30 μm