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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Anti-α-synuclein immunotherapy reduces α-synuclein propagation in the axon and degeneration in a combined viral vector and transgenic model of synucleinopathy

Fig. 6

Immunohistochemical analysis of passively immunized mice following unilateral LV-α-syn injection. α-Syn KO, non-tg, and α-syn tg mice were passively immunized weekly for 13 weeks with intraperitoneal injections of either 27-1 (control) or 1H7 (α-syn antibody) beginning one week after LV-α-syn unilateral injection. Pathological analysis was conducted 16 weeks following unilateral LV-α-syn injection. Photomicrographs are of low and high magnification, with the high magnification image identified by the boxed-inset in the low magnification images. For these experiments an antibody against total α-syn was used. a Representative photomicrographs and (b) computer-aided analysis of contra- and ipsi-lateral hippocampus immunostained with an antibody against α-syn from α-syn KO mice immunized with 27-1 (control; n = 8) or 1H7 (α-syn antibody; n = 9). c Representative photomicrographs and (d) computer-aided analysis of contra- and ipsi-lateral hippocampus immunostained with an antibody against α-syn from non-tg mice immunized with 27-1 (control; n = 8) or 1H7 (α-syn antibody; n = 9). e Representative photomicrographs and (f) computer-aided analysis of contra- and ipsi-lateral hippocampus immunostained with an antibody against α-syn from α-syn tg mice immunized with 27-1 (control; n = 8) or 1H7 (α-syn antibody; n = 8). *P-value < 0.05 using ANOVA followed by Dunnett’s post-hoc test comparing 27-1 (control) and 1H7 (antibody) within a hemisphere, and # P-value < 0.05 using ANOVA followed by Tukey-Kramer post ho test comparing contra- and ipsilateral sides. Low magnification scale bar = 100 μm. High magnification scale bar = 25 μm

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