Fig. 4

Immunofluorescent and PCR analysis of LV-DNA in the hippocampus of α-syn KO, non-tg and α-syn tg mice. a Representative immunofluorescent images with an anti-synuclein antibody (syn-211) at low and high magnification of the contra- and ipsi-lateral CA1 hippocampal regions following unilateral LV-α-syn injection. In the ipsilateral side α-syn was detected in nerve cell bodies and neurites in the hippocampus, and in the contralateral side in the molecular layer of the dentate gyrus. b By PCR LV-α-syn was detected at high levels in the ipsilateral side and at background levels in the contralateral side. c Representative fluorescent images at low and high magnification of the contra- and ipsi-lateral CA1 hippocampal regions following unilateral LV-GFP injection. GFP was detected primarily in the neuronal cell bodies in the ipsilateral but not in the contralateral side. d LV-GFP DNA was detectable above background levels in the ipsilateral but not the contralateral side in all genotypes. For all experiments, PCR expression of LV DNA was normalized to GAPDH. *P <0.05 using a Student’s t-test comparing contra- and ipsilateral sides within a genotype. N = 5 per group. Low magnification scale bar = 100 μm; high magnification scale bar = 10 μm