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Fig. 10 | Acta Neuropathologica Communications

Fig. 10

From: Anti-α-synuclein immunotherapy reduces α-synuclein propagation in the axon and degeneration in a combined viral vector and transgenic model of synucleinopathy

Fig. 10

Co-localization of microglial marker Iba-1 and α-syn was increased in passively immunized α-syn tg mice. To analyze if passive immunization was associated with microglial clearance of α-syn, double labeling of brain sections of α-syn KO, non-tg, and α-syn tg mice that were passively immunized weekly for 13 weeks with either 27-1 (control) or 1H7 (α-syn antibody) beginning one week after LV-α-syn unilateral injection were analyzed using Iba-1 antibody against microglia and α-syn (syn-211)-positive aggregates was conducted 14 weeks following unilateral LV-α-syn injection. Double labeled sections were analyzed with the laser scanning confocal microscope. Panels in red represents α-syn (syn-211 antibody), and green represents Iba-1(a) Representative confocal images of the contralateral hippocampal neuropil in brain sections from α-syn KO, non-tg, and α-syn tg mice immunized with 27-1 (control), or (b) immunized with 1H7 (antibody). Arrows indicate co-localization of the Iba-1 signal with α-syn in microglia. c Analysis of the percentage of co-localization between the microglial marker Iba-1 and the α-syn syn-211 antibody. Error bars represent ± SEM. α-Syn KO mice + 27-1 (control) n = 8; α-syn KO + 1H7 antibody n = 9; non-tg mice + 27-1 (control) n = 10; non-tg mice + 1H7 antibody n = 9; α-syn tg mice + 27-1 (control) n = 8; α-syn tg mice + 1H7 antibody n = 8. Scale bar = 10 μm

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