Table 1 Primer for target-genes used in quantitative-real-time PCR (qPCR). DRD1 primer was bought from ThermoFisher Scientific (Hs00265245_s1) as was DRD2 primer (MS00241436)
gene | forward primer | reverse primer | probe |
|---|---|---|---|
HPRT | 5′-tgaacgtcttgctcgagatgtg-3′ | 5′-ccagcaggtcagcaaagaattt-3′ | 5′-tgggaggccatcacattgtagcc-3′ |
SNCA | 5′-ccaaagagcaagtgacaaatgttg-3′ | 5′-agccagtggctgctgcaat-3′ | 5′-tgacgggtgtgacagcagtagccca-3′ |
5HT3AR | 5-ggctggtgcacaagcaagac-3′ | 5-ggctggtgcacaagcaagac-3′ | 5-ctgcttggctgcgtcacctggttct-3′ |
5HT4R | 5′-attccgggttgaacccttttc-3′ | 5′-aggtcttcggtagcgctcatc-3′ | 5′-acgtgccttcctcatcatcctct-3′ |
M3R | 5′-cagctgcatacccaaaaccttt-3′ | 5′-tgaatgttttgttgcacagagcata-3′ | 5′-caacagcaccgtgaaccccgtgtg-3′ |
VIP | 5′-aataaggcccagctccttgtg-3′ | 5′-cccaacctgagagcagaaggt-3′ | 5′-cttctcacagacttcggcatggc-3′ |