Fig. 4

Confocal analysis of co-localization of P2RY12, CD163 and CD45 in GBM. a: Upper row: overview of immunostaining for P2RY12 and CD163 in a representative GBM. White arrows indicate nuclear P2RY12 signal in cells with high CD163 signal; Cyan arrows indicate cytoplasmic P2RY12 signal in cells with low CD163 signal (scale bar:100 μm). Lower row: selected view of P2RY12 and CD163 staining in glioblastoma. White arrows indicate cells with weak P2RY12 and high CD163 signal (scale bar: 50 μm). Inserts: details of cells with low CD163 and strong cytoplasmic P2RY12 signals, and cells with strong CD163 and weak P2RY12 signals. b: Overview with two inserts. Upper panel: area with cells with nuclear P2RY12 signal with strong CD163 expression (scale bar: 50 μm). Lower panel: area with cytoplasmic P2RY12 signals and weak CD163 expression (scale bar: 50 μm). White arrows: nuclear P2RY12 expression with strong CD163 signals. c: Overview: triple staining for CD163, P2RY12 and CD45 in GBM (scale bar: 50 μm). Inserts (scale bar: 20 μm): Cells with high CD163 and nuclear P2RY12 signals did not overlap with CD45. d: Confocal images showing triple staining for CD163, P2RY12 and CD45 in GBM (scale bar: 20 μm). Cells with high CD163 and nuclear P2RY12 signal did not overlap with CD45