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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Endosulfine-alpha inhibits membrane-induced α-synuclein aggregation and protects against α-synuclein neurotoxicity

Fig. 4

WT ENSA (but not the S109E mutant) alleviates aSyn neurotoxicity. Primary midbrain cultures were transduced with adenovirus encoding A30P (a, c, e) or G51D (b, d) at an MOI of 10, in the absence or presence of adenovirus encoding WT ENSA, S109E, or the control protein β-gal (LacZ) at an MOI of 5. Additional cultures were untransduced (‘control’) or transduced with LacZ adenovirus alone. The cells were fixed, stained with antibodies specific for MAP2 and TH, and scored for dopaminergic cell viability (a, b) or neurite lengths (c, d). The data are presented as the mean ± SEM, n = 3. (a, b) **p < 0.01, ***p < 0.001, ****p < 0.0001 versus control or LacZ, ## p < 0.01, ### p < 0.001 versus WT ENSA; square root transformation, one-way ANOVA followed by Tukey’s multiple comparisons post hoc test. (c, d) ****p < 0.0001, Tukey’s multiple comparisons post hoc test after general linear model implementation. (e) Representative fluorescence images showing the more pronounced retraction of neurites extending from MAP2+/TH+ neurons in cultures expressing A30P alone or A30P with ENSA S109E compared with untransduced cultures, cultures expressing β-gal, or cultures expressing A30P with WT ENSA (scale bar, 50 μm)

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