Fig. 3

WT ENSA (but not the S109E mutant) interferes with vesicle disruption by A30P and G51D. Calcein-loaded SUVs were incubated in the absence (Control) or presence of A30P (a, c) or G51D (b, d) (40 μM of each), with or without WT ENSA (10–40 μM) (a, b), or with or without WT ENSA or S109E (40 μM) (c, d). Calcein release was monitored via fluorescence measurements at an excitation wavelength of 485 nm and an emission wavelength of 515 nm. The data are presented as % dye release versus time, with 100% release determined as the signal obtained from vesicles treated with Triton X-100. Mean ± SEM, n = 3 or 4. P values obtained from a statistical analysis of the data are listed in Additional file1: Tables S1 and S2