Fig. 1

WT ENSA (but not the S109E mutant) inhibits membrane-induced aggregation of A30P and G51D. A mixture of A30P (a−d) or G51D (e−h) (40 μM of each) and SUVs was incubated in the absence (−) or presence of WT ENSA (10–40 μM) (a, b, e, f) or in the absence (−) or presence of WT ENSA or S109E (40 μM) (c, d, g, h) for 72 h. Membrane fractions were isolated and analyzed via Western blotting. (a, c, e, g) Representative Western blot images (‘olig’ refers to the region of the blot corresponding to high molecular weight forms of aSyn). (b, d, f, h) Bar graphs showing the ratio of total oligomer intensity to monomer intensity determined via densitometric analysis. The data are presented as the mean ± SEM, n = 4; *p < 0.05, **p < 0.01, ***p < 0.001 versus control (−); ^# p < 0.05 versus ‘ENSA, 10 μM’, one-way ANOVA followed by Tukey’s multiple comparisons post hoc test