Fig. 3
From: The effects of the novel A53E alpha-synuclein mutation on its oligomerization and aggregation
A53E reduces aSyn oligomerization. a Fluorescent cells, expressing VN-aSyn and aSyn-VC constructs, as a result of the aSyn interaction. Scale bar: 30 μm. b Mean fluorescence intensity of cells were assessed 24 h post-transfection using an Olympus IX81-ZDC microscope. For each condition, 100 pictures were acquire in 4 independent experiments were conducted. Student’s t test (**p < 0.01). c-d aSyn protein levels were assessed by immunoblot analysis and were found to similar between WT aSyn and the A53E mutant. n = 3. e Native-PAGE gel showed that the A53E mutant forms high molecular weight species similar to WT aSyn