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Fig. 8 | Acta Neuropathologica Communications

Fig. 8

From: Dopamine-2 receptor extracellular N-terminus regulates receptor surface availability and is the target of human pathogenic antibodies from children with movement and psychiatric disorders

Fig. 8

Comparison of antibody binding to four full-length D2R mutants. Binding was similar between N23Q and N23Q/D26E/A29P mutants with WT D2R (100% binding shown as dotted line). Murinization of amino acids 26 and 29 (D26E/A29P) strongly reduced binding. Interestingly, L R20K/P21G/F22W/N23Q/D26E/A29P mutant had the greatest impact on antibody binding. Percentage of sera binding to D2R mutants (MFI %) was calculated using the formula described in Material and Methods. Results are expressed as mean ± SD %. Data was analyzed by one-way ANOVA analysis of variants with a Dunn’s multiple comparison test, *P < 0.05, **P < 0.01, ***P < 0.001

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