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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Dopamine-2 receptor extracellular N-terminus regulates receptor surface availability and is the target of human pathogenic antibodies from children with movement and psychiatric disorders

Fig. 1

Patient anti-D2R IgG antibodies induce receptor internalization and decrease D2R surface density. a, b Anti-D2R antibody-positive movement and psychiatric disorder (MPD, n = 3) protein G-purified IgG from patients with similar ΔMFIs (14,388; 14,789; and 18,740, Fig. 2e) induces a downregulation of surface D2R on live cells compared to IgG purified from control (CTL, n = 2), other neurological disorders (OND, n = 2), and demyelinating diseases (DEM, n = 2). c Anti-D2R antibody-positive MPD (n = 3) protein G-purified IgG-incubated on live cells have a similar expression of cytoplasmic GFP compared to IgG purified from CTL (n = 2), OND (n = 2), and DEM (n = 2). d, e MPD protein G-purified IgG and IgG purified from CTL have no effect on surface NCAM (expressed in all HEK293 cells). Representative images are shown (volume projection of entire Z-stack). Nuclei stained with dapi (scale bar = 25μm). 80 different cells (1 cell = 1 diamond) for each control and patient out of three independent experiments are shown. Results are expressed as fluorescence intensity relative to culture medium (100%) after normalization to GFP and red bars represent mean. Data was analyzed by Mann-Whitney U test, *P < 0.0001

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