Fig. 2

Differences in the monocyte subset ratio of human LRRK2 overexpressing mice. a, b Ly6C^high and Ly6C^low monocyte subsets of mouse models for PD were analyzed by six-color flow cytometry. a A significant increase in the ratio of Ly6C^high to Ly6C^low monocyte subsets was detected in 20 month old mutant LRRK2^(R1441G) BAC transgenic mice in comparison to NT littermates. b A trend of an increasing ratio of Ly6C^high to Ly6C^low monocytes of LRRK2 WT-OX mice compared to NT littermates were identified in 20 month old animals. Error bars represent mean ± SEM, *p < 0.05; ns: not significant; statistical significance was tested with 2way ANOVA (c) Murine PBMCs were isolated from whole blood samples and isolated RNA was transcribed into cDNA. PCR products were visualized on a 2% agarose-gel. A band with 153 bp represent human LRRK2 and is only detected in PBMCs from LRRK2 WT-OX mice and not in NT littermates; −RT: negative reverse transcription control, NT: non-transgenic