Fig. 6

Neuron-to-neuron transfer of Tau fibrils through TNTs. For (a) and (b), cells were infected with LVs encoding GFP-Actin (red). At 48 h post-infection, cells were incubated for six hours with 1 μM ATTO 568-hTau1N4R fibrils (green). a Snapshots selected from a time-lapse video and placed in a gallery to visualize neuron-to-neuron transfer of extracellular Tau fibrils by TNTs in neuronal CAD cells. Time-lapse videos were acquired during 5 h and 33 min with an inter-image interval of 6 s. b Images selected from a video to visualize transfer of extracellular hTau1N4R fibrils by TNTs in primary neurons. Neurons were imaged every 6 s for 51 min. For (a) and (b), cells were filmed with an inverted spinning disk microscope using a 63× oil-immersion lens (NA 1.4) and processed with ZEN blue and ImageJ software. For time-lapse acquisition, a focal plane was collected for each specimen. Scale bars: 10 μm