Fig. 4

Intracranial injection of H-Aβ induces behavioral defects in wt mice. a–c Mice hyperlocomotion, assessed by Open Field test. Left panel a total distance (cm) travelled by mice in 30 min using 5 min time bin representation. b, c panels: total distance travelled in 30 min in the periphery or in the center of the arena using 5 min time bin representation. Statistical analysis was performed by One way Anova, Bonferroni multiple comparison test. Asterisks are referred to the vehicle vs H-Aβ42/H-Aβ24 mix condition. No significant differences are present between APP/PS1 tested mice and the H-Aβ42/H-Aβ24 mix condition. (****P < 0.0001; ***P < 0.001; **P < 0.01). d–f Social interaction, assessed using the social free test. Graphic representation of the number of contacts/min between the two mice in the same arena. H-Aβ42 d, H-Aβ24 e or H-Aβ42/H-Aβ24 mix f are compared to vehicle injected mice and APP/PS1 mice. Statistical analysis was performed by One way Anova, Bonferroni multiple comparison test (**P < 0.01; *P < 0.05). g and h Assessment of learning and memory by NOR. g Number of contacts with the new and old object either after 1 h and 24 h of delay. h Number of contacts with the new object both after 1 h and 24 h of delay. Statistical analysis was performed by One way Anova, Bonferroni multiple comparison test. Asterisks are referred to the control wt vs APP/PS1 mice when specified or to the vehicle vs other conditions (****P < 0.0001; **P < 0.01; *P < 0.05). A total of 33 mice (6 mice for each experimental group plus 6 APP/PS1 and 3 wt mice 6 months old) were analysed