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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Amyloid-β 1–24 C-terminal truncated fragment promotes amyloid-β 1–42 aggregate formation in the healthy brain

Fig. 1

Intracranial injection of H-Aβ24 and H-Aβ42/H-Aβ24 mix in wt mice induces amyloid aggregate deposition. a 6E10 DAB staining of hippocampal brain sections 6 months after vehicle, H-Aβ42, H-Aβ24 or H-Aβ42/H-Aβ24 mix injection. Sagittal plane sections are shown on the left; middle and right panels show enlargements of the dorsal cerebral cortex. 6E10-immunopositive aggregates are visible on a cresyl violet-luxol fast blue counterstaining. Scale bars: left 1 mm, middle 100 μm, right 50 μm. b 6E10 DAB staining of brain sections 2.5 months after vehicle, H-Aβ42, H-Aβ24 or H-Aβ42/H-Aβ24 mix injection. Scale bar: 50 μm. c Congo red staining of brain sections 2.5 months after the injection of vehicle, H-Aβ42, H-Aβ24 or H-Aβ42/H-Aβ24 mix. On the right, representative images of aggregates detected 6 months after H-Aβ42/H-Aβ24 mix injection are shown. Top: bright field images; bottom: epifluorescence images -FITC filter. Scale bar: 50 μm. d and e Quantification of 6E10 DAB positive plaques 2.5 or 6 months after the injection of vehicle, H-Aβ42, H-Aβ24 or H-Aβ42/H-Aβ24 mix. 6 brain sections were analyzed for each mice (N = 3 mice for each group). Statistical analysis was performed by One way Anova, Bonferroni multiple comparison test (****P < 0.0001; ***P < 0.001). f and g Quantification of Congo red-positive plaques area (μm2) per brain section at 2.5 or 6 month after injection. 10 sections of 50 μm thickness per slice were analyzed for each mouse brain (N = 6 mice for each group). Statistical analysis was performed by One way Anova, Bonferroni multiple comparison test (**P < 0.01). h Cartoon depicting the experimental scheme

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