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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Tor1a+/- mice develop dystonia-like movements via a striatal dopaminergic dysregulation triggered by peripheral nerve injury

Fig. 4

Dopamine metabolism is disturbed in Tor1a+/- mice. a Representative Western blot of striatal DAT, DA D1 and DA D2 receptors in naïve wt, naïve Tor1a+/, crush injured wt and crush injured Tor1a+/- mice. GAPDH is used as loading control. b Relative DAT, (h) DA D1 receptor and (i) DA D2 receptor protein levels in striatum contralateral to crush injury are shown (mean ± SD) comparing wt (blue) and Tor1a+/- (orange) mice before and 8 weeks after crush injury. c Diagram demonstrates relative DAT mRNA expression by real-time PCR in midbrain contralateral to crush injury in wt and Tor1a+/- mice before and 8 weeks after sciatic nerve crush (mean ± SD). d Representative images of in-vivo DAT autoradiography with FP-CIT in Tor1a+/- control (left) and crush injured mutant (right). e Diagram shows mean striatal DAT binding counts in wt and Tor1a+/- mice before and 8 weeks after sciatic nerve crush (mean ± SD). f, g Relative striatal DA and HVA levels measured by HPLC are shown in wt and Tor1a+/- mice before and 8 weeks after crush injury contralateral to nerve crush (mean ± SD). j, k Ipsilateral DA D1 and DA D2 receptor relative protein levels are demonstrated in wt and Tor1a+/- mice before and 8 weeks after crush injury (mean ± SD). n = number of mice are depicted below the diagrams (wt/Tor1a+/-). Statistical analysis was performed by using the parametric one-way ANOVA with posthoc Tukey test. *p < 0.05, **p < 0.01, ***p < 0.001

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