Fig. 6

DUSP26 and PTPRT suppress glioblastoma cell growth and motility. DUSP26 (a) and PTPRT (b) mRNA levels in lentivirally transduced E98 cells were determined by qRT-PCR. Expression, normalized to ACT-B, is given as fold change of that in GFP control cells. c Transduced E98 cells were grown on coverslips for 48 h, pulse-labeled with EdU for 1 h, fixed, and EdU incorporation was visualized. Representative images of DAPI, EdU and TagRFP positivity are depicted. d Percentage of EdU-positive nuclei among DAPI-stained cells was determined (n > 3). Error bars indicate SD and asterisks reflect confidence levels (ANOVA; ** p < 0.01; *** p < 0.001). e Spheroids of transduced E98 cells on a confluent mouse astrocyte layer were incubated for 24 h before being fixed. Representative images of TagRFP-expressing spheroids with outgrowing cells are shown for the respective constructs. f The average radius increase (in pixels) due to migrating cells was calculated per spheroid from the data in (e), and plotted as gray data points on top of the box plot for the mean with standard deviation. Asterisks indicate significance (*** p < 0.001; Kruskal-Wallis one way analysis of variance p < 0.0001)