Skip to main content
Fig. 8 | Acta Neuropathologica Communications

Fig. 8

From: Developmental delay in motor skill acquisition in Niemann-Pick C1 mice reveals abnormal cerebellar morphogenesis

Fig. 8

Oligodendrocyte maturation is impaired in Npc1 nmf164 mice. a Western blot analysis of MBP protein expression in cerebella of PN11 and PN15 wt and Npc1 nmf164 mice. Histograms indicate the abundance (mean ± SEM) of each isoform determined by densitometry of protein bands obtained in at least 3 independent experiments taking β-actin as internal reference. * p ≤ 0.01, *** p < 0.0001. b Immunostaining with antibodies directed to MBP (brown) shows that PN15 Npc1 nmf164 mouse cerebella display a reduction of MBP expression at the level of PC axons and white matter compared to wt littermates. Representative fields of parasagittal sections of lobule III of PN15 wt and Npc1 nmf164 mouse cerebella are shown in the Fig.; scale bars: 100 μm. c Immunostaining with antibodies directed to MBP (brown) showing that PN15 Npc1 nmf164 mice display a poorer oligodendrocyte differentiation as indicated by the reduced length of MBP-positive processes that typically radiate from oligodendrocyte soma (arrowheads), compared to wt littermates. Representative fields of parasagittal sections of PN15 wt and Npc1 nmf164 mouse cerebral cortex (Cx) and splenium of corpus callosum (scc) are shown in the Fig.; scale bars: 100 μm. Higher magnifications are shown in panel C (bottom); scale bars: 20 μm

Back to article page
\