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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Developmental delay in motor skill acquisition in Niemann-Pick C1 mice reveals abnormal cerebellar morphogenesis

Fig. 7

Purkinje cells of Npc1 nmf164 mice display a reduced number of GABAergic inputs. a Immunostaining with antibodies directed to GAD65 (brown) shows a reduced density of GAD65-positive puncta (arrowheads) around Purkinje cell soma and throughout the entire molecular layer of PN15 Npc1 nmf164 mice compared to wt littermates. Representative fields of parasagittal sections of lobule II wt and Npc1 nmf164 mice cerebella are shown in the Fig.; scale bars: 10 μm. Higher magnifications are shown in bottom panel insets; Arrowheads indicate typical GAD65-positive puncta; scale bars: 10 μm. Histograms indicate the density of GAD65-positive puncta in outer and inner molecular layers and Purkinje cell layer. b Western blot analysis of GAD65 protein expression in cerebella of PN15 wt and Npc1 nmf164 mice. Histograms indicate GAD65 abundance (mean ± SEM) determined by densitometry of protein bands obtained in at least 3 independent experiments taking the β-actin as internal reference. * p = 0.01

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