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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Developmental delay in motor skill acquisition in Niemann-Pick C1 mice reveals abnormal cerebellar morphogenesis

Fig. 6

Purkinje cells of Npc1 nmf164 mice display a reduced number of glutamatergic inputs. a Immunostaining with antibodies directed to VGluT2 (brown) shows that PN15 Npc1 nmf164 mice display a reduced expression of VGluT2 in the outer part of molecular layer compared to wt littermates. Representative fields of parasagittal sections of lobule II of wt and Npc1 nmf164 mouse cerebella are shown. Upper panels: arrows indicate VGluT2-positive synapses of internal granule layer glomeruli; scale bars: 20 μm. Bottom panels: higher magnifications of selected areas. Arrowheads indicate typical VGluT2 positive puncta; scale bars: 5 μm. Histograms indicate VGluT2-positive puncta densities in the outer and inner molecular layers (mean ± SEM). b Western blot analysis of VGluT2 protein expression in cerebella of PN15 wt and Npc1 nmf164 mice. Histograms indicate VGluT2 abundance (mean ± SEM) determined by densitometry of protein bands obtained in at least 3 independent experiments taking the β-actin as internal reference. * p < 0.01

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