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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Developmental delay in motor skill acquisition in Niemann-Pick C1 mice reveals abnormal cerebellar morphogenesis

Fig. 5

Bergmann glia function appears to be defective in Npc1 nmf164 mice. a Immunostaining with antibodies directed to GLAST (brown) shows that PN15 Npc1 nmf164 mice display a reduced expression of GLAST at the level of BG processes in the outer part of molecular layer (arrowheads) and around Purkinje cell soma (arrows) compared to wt littermates. Representative fields of parasagittal sections of wt and Npc1 nmf164 mouse cerebella are shown in the Fig.; scale bars: 10 μm. Higher magnification fields are shown on the right; scale bars: 5 μm. b Immunostaining with antibodies directed to Glutamine synthetase (brown) shows that PN15 Npc1 nmf164 mice display a reduced expression of Glutamine synthetase at the level of BG soma (arrowheads) and processes compared to wt littermates. Representative fields of parasagittal sections of wt and Npc1 nmf164 mouse cerebella are shown in the Fig.; scale bars: 20 μm. Higher magnification fields are shown on the right; scale bars: 5 μm. ML: Molecular Layer; PCL: Purkinje Cell Layer. c-d Western blot analyses of GLAST (c) and Glutamine synthetase (d) protein expression in cerebella of PN15 wt and Npc1 nmf164 mice. Histograms indicate GLAST (c) and Glutamine synthetase (d) abundance (mean ± SEM) determined by densitometry of protein bands obtained in at least 3 independent experiments taking the β-actin as internal reference. * p < 0.01

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