Fig. 4

Bergmann glia morphogenesis is defective in Npc1 ^nmf164 mice. a Immunostaining with antibodies directed to GFAP (brown) shows that BG of PN15 Npc1 ^nmf164 mice have radial shafts that are enlarged and irregular in caliber, as well as hypertrophic astrocytes within the IGL, compared to wt littermates. Representative fields of parasagittal sections of wt and Npc1 ^nmf164 mouse cerebella are shown in the Fig.; scale bars: 50 μm. Higher magnification fields are shown on the right; scale bars: 25 μm. ML: Molecular Layer; PCL: Purkinje Cell Layer; IGL: Internal Granular Layer. b Western blot analysis of GFAP protein expression in cerebella of PN15 wt and Npc1 ^nmf164 mice. Histograms indicate the abundance (mean ± SEM) of each GFAP isoforms determined by densitometry of protein bands obtained in at least 3 independent experiments taking β-actin as internal reference. * p ≤ 0.01