| Cleaved Caspase-8 | Cleaved Caspase-3 | CD68 upregulated | Days after stroke |
---|
Case 1 | ++ | ++ | Yes | 0–1 |
Case 2 | ++ | + | Yes | 0–1 |
Case 3 | + | + | Yes | 0–1 |
Case 4 | + | + | Yes | 1–4 |
Case 5 | + | – | Yes | 1–4 |
Case 6 | + | – | Yes | 5–29 |
Case 7a | + | – | No | 5–29 |
Case 8 | + | – | No | 5–29 |
Case 7b | – | – | No | 30– |
Case 9 | – | – | No | 30– |
Control 1 | – | – | No | |
Control 2 | – | – | No |
Control 3 | – | – | No | |
Control 4 | – | – | No |
Control 5 | – | – | No |
- Tissue from 10 different areas from stroke subjects (Case; n = 9) and 5 areas from healthy controls (n = 5) were analyzed for CD68, cleaved caspase-8 and cleaved caspase-3 expression by immunohistochemistry (exemplified in Fig. 4a). Cleaved caspase-8 or -3 expression levels were scored as (-) not present, (+) present or (++) high levels. CD68 expression was represented as (No) not present/basal levels or (Yes) increase in CD68 positive cells. Age of stroke area was determined by hematoxylin and eosin staining and is presented as days after stroke event. Highest levels of cleaved caspase-8 and-3 expressions were found within the first days after stroke. They were found to decrease with time, and were completely gone within 30 days. CD68-positive cells can be found at high numbers within the first days after stroke and decrease to basal levels within 30 days after stroke onset