Fig. 2
From: Diminishing evidence for torsinA-positive neuronal inclusions in DYT1 dystonia
Cellular distribution of torsinA and ChAT in DYT1 and control striatum. a Normal staining pattern and cellular distribution of torsinA in the human striatum (scale bar, 20 μm). TorsinA shows granular cytoplasmic staining (inset, arrowheads; scale bar, 10 μm). b, c, d Fluorescence-like multispectral image (gated immunohistochemical chromogen signal) of torsinA protein (red) reveals circumscribed collections in the cytoplasm of both DYT1 and control neurons (arrowheads; scale bars, 20 μm). ChAT (green) shows diffuse immunoreactivity within the cytoplasm and processes in both DYT1 and controls. e This section of an unaffected control shows an inclusion-like aggregate immunoreactive to torsinA (red) (scale bar, 20 μm). f, g Also identified was perinuclear accumulation of torsinA (red) seen in DYT1 tissue, thought to be a response to cellular stress (scale bars, 20 μm)