Fig. 7

Comparison of pSer129 antibody IHC staining using naïve αS transgenic and WT mice. Representative images of IHC staining of brainstem tissue from a 7 month old non-symptomatic homozygous M83 mouse (M83 unimpaired), a 12 month old motor impaired homozygous M83 mouse (M83 motor impaired) and a WT mouse. All antibodies stained perikaryal and neuritic inclusions in the M83 diseased mouse. LS7 showed weaker reactivity to αS pathology than the other antibodies and showed stronger diffused signal in the αS transgenic mice compared to the WT mouse. LS3-2C2 also showed weak staining of pathology. In addition to the pathology in motor impaired M83 mouse, antibodies 4F8, LS3-2C2, LS4-2C3 and 81A also labeled neuronal projections even in WT mice (arrowheads). EP1536Y exhibited some nuclear staining in some mouse sections (arrows), as did LS3-2C2, but much weaker than EP1536Y (eg. see arrows in the M83 unimpaired mouse). Scale bar = 50 μm