Fig. 6

Comparison of novel antibodies in detecting pathological inclusions in αS transgenic mice injected with αS fibrils in the periphery (intramuscular) or the brain (hippocampus). Representative images of IHC staining of tissue from M83 αS transgenic mice injected in the gastrocnemius muscle, and M83 and M20 αS transgenic mice injected in the hippocampus with recombinant preformed αS fibrils. Images were taken from the brainstem (muscle injection) and the hippocampus (hippocampal injection). Antibodies EP1536Y and 81A showed robust staining of induced inclusions (arrows). LS7 stained inclusions weakly with higher general labeling. Novel antibodies raised against the pSer129 αS epitope LS4-2G12, LS3-2C2 and LS4-2C3 or to the p473 NFL epitope all stained the induced inclusions in these models. Scale bar = 50 μm