Fig. 5

Characterization of the specificity of novel pSer129 αS antibodies by immunoblotting analyses using total lysates of cortex and brainstem/spinal cord tissues from WT and NFL null mice. Cerebral cortex and brainstem/spinal cord (BS/SC) from a WT, an NFL null (NFL KO) and an αS null (αS KO) mouse were dissected and lysed in 2 % SDS/ 50 mM Tris pH 7.5 as described in “Materials and methods”. Equal amounts of proteins (5 μg) from each sample was resolved onto 10 % polyacrylamide gels and analyzed by immunoblotting with each antibody indicated above. The protein band identified by an asterisk depicts NFL. The mobility of molecular mass markers are shown on the left