Fig. 3

Characterization of the specificity of novel pSer129 αS antibodies by immunoblotting analyses using biochemically fractionated brainstem/spinal cord mouse tissues. Brainstem/spinal cord from an αS null (αS KO), a WT, a 2 month old non-symptomatic M83 (M83) and a 12 month old motor impaired M83 (M83-I) mouse were biochemically fractionated into high salt (HS), high salt/Triton X-100 (HS/T) and SDS/urea (SDS/U) fractions as described in “Materials and methods”. 10 μg of HS and HS/T, and 5 μg of SDS/U fractions were resolved onto 13 % polyacrylamide gels and analyzed by immunoblotting with each antibody indicated above. The protein band identified by an arrowhead is αS, while an asterisk depicts NFL. The mobility of molecular mass markers are shown on the left