Fig. 10

Analysis of the induction of endogenous αS aggregation by treatment with exogenous αS mouse fibrils in primary neuronal-glial cultures using antibody LS4-2G12. Primary neuronal-glial cultures from WT mice or αS null mice were cultured for 6 days and either maintained without other treatment for 8 days (Ct) or treated with mouse αS fibrils (20 μg/ml; αS Fib) for 8 days. Double immunofluorescence analysis with antibodies LS4-2G12 (red) and specific neuronal marker βIII-tubulin (green) was performed. Cells were also counterstained with DAPI and merged images are shown. Higher magnification LS4-2G12 and merged images are shown on the far right. Arrows depict induced labeled αS aggregates. Scale bar = 100 μm and 250 μm for the higher magnification images on the right