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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Novel antibodies to phosphorylated α-synuclein serine 129 and NFL serine 473 demonstrate the close molecular homology of these epitopes

Fig. 1

In vitro phosphorylation of NFL and αS by CKII and PLK1, 2 and 3. a Amino acid sequences surrounding the target phosphorylation sites (bold) of Ser129 for αS, and Ser473 for NFL. b Quantitative analysis of the stoichiometric phosphorylation of recombinant αS, S129A αS, NFL and S473A NFL in in vitro kinase reactions as described in “Materials and methods” with CKII, PLK1, PLK2 or PLK3. All reactions were performed in triplicate. c Immunoblotting analyses of NFL and S473A NFL used in in vitro kinase reactions with CKII, PLK1, PLK2 or PLK3. Proteins were resolved onto 8 % polyacrylamide gel and probed with 4F8 (pSer473 NFL) and NR4 (anti-NFL) antibodies. d Immunoblotting analyses of αS and S129A αS used in in vitro kinase reactions with CKII, PLK1, PLK2 or PLK3. Proteins were resolved onto 13 % polyacrylamide gels and probed with 81A (pSer129 αS) and Syn 204 (anti-αS). The mobility of molecular mass markers are shown on the left

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