Fig. 5

Neuronal gangliosides mediate acute ADDL-induced interaction of IR and caveolin-1 and subsequent loss of surface IR in mHippoE-14 neurons. a PLAs have been performed on non-permeabilized cells. Acute ADDL exposure leads to loss of IR from the cellular surface (upper graph). Simultaneously, ADDLs induce complex formation between IR and ganglioside GD1a (middle graph). However, ADDLs do not induce IR/GM1 interactions (lower graph; surface IR: n = 150–170 cells, IR/GD1a: n = 42–46 cells, IR/GM1: n = 27–30 cells). b The PLA shows that ADDLs stimulate complex formation between IR and caveolin-1. Increased ADDL-induced complex formation is not evident in GENZ-treated cells (n = 66–94 cells). c A PLA using two IR antibodies (N-20 and D-17) shows that surface IR are not reduced on GENZ-treated cells that have been acutely exposed to ADDLs (n = 149–170 cells). Unpaired two-tailed student’s t-test (p ≤ 0.05 is marked with (*)); 5 μM ADDLs, 30 min. Scale bars: 10 μM