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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Isoaspartic acid is present at specific sites in myelin basic protein from multiple sclerosis patients: could this represent a trigger for disease onset?

Fig. 7

Oxidation of Met22 coupled with racemization of Asp23 in MBP from controls () and multiple sclerosis (MS) patients suffering from SPMS () PPMS () and RRMS (). a When all Asp versions of (YLATASTMDHAR) were included, Met oxidation in the controls was significantly greater than in multiple sclerosis patients (p < 0.001, Mann–Whitney-U). b Oxidation of Met22 and racemization of Asp23 to isoAsp in YLATASTMDHAR. In the Met-oxidized peptide, racemization of Asp23 (YLATAST(MetSO)(isoAsp)HAR) was greater in the control samples (p = 0.008, Mann–Whitney-U). IsoAsp in this case refers to combined D- and L-isoAsp, since the isomers were not separated under these conditions. c Racemization of Asp23 to isoAsp(YLATASTM(isoAsp)HAR) in the absence of Met oxidation. Levels of isoAsp were significantly higher in multiple sclerosis patients (p < 0.001, Mann–Whitney-U). The percentage of modification was determined by the ion intensities of (Modified YLATASTMDHAR)/(YLATASTMDHAR + YLATASTMDHAR) × 100. Controls n = 10; multiple sclerosis patients n = 8

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