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Table 6 Morphometric dendritic and dendritic spine parameters of LII-IIIPNs within the somatosensory cortex. All data presented as mean ± SEM

From: Marked changes in dendritic structure and spine density precede significant neuronal death in vulnerable cortical pyramidal neuron populations in the SOD1G93A mouse model of amyotrophic lateral sclerosis

Region

P8-15 (n) Neonatal

P28-35 (n) Pre-symptomatic

P65-75 (n) Onset

P120 (n) Mid-disease

Soma volume (μm3)

WT: 749.7 ± 139.9 (7)

WT: 1051.6 ± 149.9 (5)

WT: 1119.7 ± 54.4 (5)

WT: 929.9 ± 281.3 (8)

SOD1: 954.5 ± 261.2 (5)

SOD1: 1127.8 ± 304.3 (5)

SOD1: 823.8 ± 96.4 (5)

SOD1: 853.8 ± 192.9 (12)

Not significant

Not significant

Not significant

Not significant

Total arbor length (μm)

WT: 2355 ± 366 (7)

WT: 2408 ± 324 (5)

WT: 2410 ± 323 (5)

WT: 2869 ± 311 (8)

SOD1: 2044 ± 416 (5)

SOD1: 2405 ± 402 (5)

SOD1: 2466 ± 483 (5)

SOD1: 2176 ± 187 (12)

Not significant

Not significant

Not significant

Not significant

Apical arbor length (μm)

WT: 1081 ± 115 (7)

WT: 1172 ± 187 (5)

WT: 1186 ± 172 (5)

WT: 1654 ± 207 (8)

SOD1: 1020 ± 162 (5)

SOD1: 949 ± 103 (5)

SOD1: 1000 ± 231 (5)

SOD1: 1225 ± 96 (12)

Not significant

Not significant

Not significant

Not significant

Basal arbor length (μm)

WT: 1317 ± 236 (7)

WT: 1236 ± 149 (5)

WT: 1224 ± 154 (5)

WT: 1159 ± 124 (8)

SOD1: 968 ± 277 (5)

SOD1: 1455 ± 369 (5)

SOD1: 1466 ± 267 (5)

SOD1: 951 ± 119 (12)

Not significant

Not significant

Not significant

Not significant

Mean basal tree length (μm)

WT: 389 ± 47 (7)

WT: 335 ± 48 (5)

WT: 313 ± 32 (5)

WT: 325 ± 38 (8)

SOD1: 293 ± 102 (5)

SOD1: 364 ± 95 (5)

SOD1: 341 ± 38 (5)

SOD1: 235 ± 29 (12)

Not significant

Not significant

Not significant

Not significant

Apical reach (μm)

WT: 323 ± 23 (7)

WT: 486 ± 65 (5)

WT: 501 ± 66 (5)

WT: 528 ± 56 (8)

SOD1: 463 ± 67 (5)

SOD1: 367 ± 12 (5)

SOD1: 455 ± 61 (5)

SOD1: 468 ± 57 (12)

Not significant

Not significant

Not significant

Not significant

Basal reach (μm)

WT: 202 ± 18 (7)

WT: 234 ± 30 (5)

WT: 236 ± 22 (5)

WT: 207 ± 13 (8)

SOD1: 205 ± 51 (5)

SOD1: 264 ± 48 (5)

SOD1: 239 ± 35 (5)

SOD1: 196 ± 22 (12)

Not significant

Not significant

Not significant

Not significant

Apical ramifications

WT: 6.8 ± 1.2 (7)

WT: 5.7 ± 0.7 (5)

WT: 7.2 ± 0.5 (5)

WT: 9.2 ± 1 (8)

SOD1: 8 ± 0.7 (5)

SOD1: 5.3 ± 0.5 (5)

SOD1: 5.5 ± 1.2 (5)

SOD1: 5.2 ± 0.5 (12)

Not significant

Not significant

Not significant

Not significant

Basal ramifications

WT: 4.3 ± 0.4 (7)

WT: 3.8 ± 0.4 (5)

WT: 4.1 ± 0.1 (5)

WT: 4.3 ± 0.3 (8)

SOD1: 3.7 ± 0.4 (5)

SOD1: 5.1 ± 1 (5)

SOD1: 4.3 ± 0.4 (5)

SOD1: 3.7 ± 0.2 (12)

Not significant

Not significant

Not significant

Not significant

Apical spine density per 100 μm

WT: 30.5 ± 1.4 (7)

WT: 31.6 ± 3.1 (5)

WT: 34 ± 2.1 (5)

WT: 25.8 ± 3.4 (8)

SOD1: 25.5 ± 3.1 (5)

SOD1: 32.8 ± 4.4 (5)

SOD1: 28.2 ± 6.6 (5)

SOD1: 24.6 ± 2.5 (12)

Not significant

Not significant

Not significant

Not significant

Basal spine density per 100 μm

WT: 21.6 ± 2.5 (7)

WT: 29.6 ± 6.9 (5)

WT: 24.2 ± 1.7 (5)

WT: 17.4 ± 2.1 (8)

SOD1: 23.6 ± 4.7 (5)

SOD1: 22.2 ± 3.1 (5)

SOD1: 18.8 ± 4 (5)

SOD1: 16.4 ± 1.5 (12)

Not significant

Not significant

Not significant

Not significant

  1. All analyses were unpaired two-way ANOVAs for age and genotype of LII-IIIPNs from the somatosensory cortex comparing soma volume (Age: P = 0.5903; Genotype: P = 0.8586), total dendritic arbor length (Age: P = 0.7853; Genotype: P = 0.3398), apical dendritic arbor length (Age: P = 0.0271*; Genotype: P = 0.0643), basal dendritic arbor length (Age: P = 0.3671; Genotype: P = 0.8741), mean basal dendritic tree length (Age: P = 0.5589; Genotype: P = 0.6085), apical dendritic reach (Age: P = 0.2468; Genotype: P = 0.6187), basal dendritic reach (Age: P = 0.2883; Genotype: P = 0.7704), apical branch ramifications (Age: P = 0.0493*; Genotype: P = 0.1262), basal branch ramifications (Age: P = 0.7336; Genotype: P = 0.8754), apical dendritic spine density per 100 μm (Age: P = 0.1546; Genotype: P = 0.2195) and basal dendritic spine density per 100 μm (Age: P = 0.0328*; Genotype: P = 0.2041). Bonferroni post-tests were used to compare the effect of genotype at each age group, with the adjusted P-value (adj. P) reported in the table for parameters that had significant genotype effects. Each animal used (n) contains mean values from multiple individual neurons, specifically P8-15 (WT: 19 neurons; SOD1: 8 neurons), P28-35 (WT: 9 neurons; SOD1: 10 neurons), P65-75 (WT: 10 neurons; SOD1: 11 neurons) and P120 (WT: 16 neurons; SOD1: 24 neurons)
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Acta Neuropathologica Communications

ISSN: 2051-5960

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