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Table 5 Morphometric dendritic and dendritic spine parameters of LVPNs within the somatosensory cortex. All data presented as mean ± SEM

From: Marked changes in dendritic structure and spine density precede significant neuronal death in vulnerable cortical pyramidal neuron populations in the SOD1G93A mouse model of amyotrophic lateral sclerosis

Region

P8-15 (n) Neonatal

P28-35 (n) Pre-symptomatic

P65-75 (n) Onset

P120 (n) Mid-disease

Soma volume (μm3)

WT: 1339.7 ± 305.4 (7)

WT: 1666.5 ± 142.4 (5)

WT: 1775.5 ± 54.4 (5)

WT: 1666.3 ± 281.3 (8)

SOD1: 1273.1 ± 242.0 (5)

SOD1: 2446.7 ± 367.1 (5)

SOD1: 24377 ± 96.4 (5)

SOD1: 1432.1 ± 192.9 (12)

Not significant

Not significant

Not significant

Not significant

Total arbor length (μm)

WT: 2917 ± 522 (7)

WT: 4406 ± 84 (5)

WT: 5527 ± 134 (5)

WT: 4458 ± 389 (8)

SOD1: 3200 ± 206 (5)

SOD1: 5240 ± 492 (5)

SOD1: 5818 ± 280 (5)

SOD1: 2953 ± 296 (12)

Not significant

Not significant

Not significant

Not significant

Apical arbor length (μm)

WT: 1734 ± 336 (7)

WT: 2854 ± 191 (5)

WT: 3534 ± 315 (5)

WT: 2512 ± 121 (8)

SOD1: 2173 ± 256 (5)

SOD1: 2169 ± 143 (5)

SOD1: 3828 ± 232 (5)

SOD1: 1838 ± 224 (12)

Not significant

Not significant

Not significant

Not significant

Basal arbor length (μm)

WT: 1183 ± 196 (7)

WT: 1551 ± 136 (5)

WT: 1912 ± 254 (5)

WT: 1821 ± 323 (8)

SOD1: 1027 ± 159 (5)

SOD1: 2061 ± 400 (5)

SOD1: 2331 ± 335 (5)

SOD1: 951 ± 90 (12)

Not significant

Not significant

Not significant

Not significant

Mean basal tree length (μm)

WT: 333 ± 56 (7)

WT: 469 ± 36 (5)

WT: 461 ± 27 (5)

WT: 371 ± 53 (8)

SOD1: 326 ± 49 (5)

SOD1: 412 ± 43 (5)

SOD1: 413 ± 40 (5)

SOD1: 253 ± 25 (12)

Not significant

Not significant

Not significant

Not significant

Apical reach (μm)

WT: 564 ± 68 (7)

WT: 1213 ± 70 (5)

WT: 1265 ± 173 (5)

WT: 922 ± 70 (8)

SOD1: 720 ± 21 (5)

SOD1: 924 ± 61 (5)

SOD1: 1179 ± 197 (5)

SOD1: 803 ± 99 (12)

Not significant

Not significant

Not significant

Not significant

Basal reach (μm)

WT: 242 ± 35 (7)

WT: 340 ± 36 (5)

WT: 293 ± 19 (5)

WT: 336 ± 65 (8)

SOD1: 209 ± 18 (5)

SOD1: 319 ± 13 (5)

SOD1: 299 ± 26 (5)

SOD1: 241 ± 24 (12)

Not significant

Not significant

Not significant

Not significant

Apical ramifications

WT: 9.2 ± 0.8 (7)

WT: 10 ± 0.6 (5)

WT: 14.3 ± 1.8 (5)

WT: 10.5 ± 1 (8)

SOD1: 9.4 ± 0.6 (5)

SOD1: 12.7 ± 0.8 (5)

SOD1: 14.9 ± 1.4 (5)

SOD1: 8.4 ± 0.9 (12)

Not significant

Not significant

Not significant

Not significant

Basal ramifications

WT: 4.1 ± 0.3 (7)

WT: 4.2 ± 0.5 (5)

WT: 4.4 ± 0.3 (5)

WT: 4.5 ± 0.5 (8)

SOD1: 4.2 ± 0.5 (5)

SOD1: 4.1 ± 0.4 (5)

SOD1: 4.5 ± 0.2 (5)

SOD1: 3.4 ± 0.2 (12)

Not significant

Not significant

Not significant

Not significant

Apical spine density per 100 μm

WT: 28.9 ± 3.1 (7)

WT: 29.4 ± 3.2 (5)

WT: 32.8 ± 2.8 (5)

WT: 25.7 ± 2.1 (8)

SOD1: 23.1 ± 3.9 (5)

SOD1: 19.4 ± 3.3 (5)

SOD1: 20.9 ± 2.4 (5)

SOD1: 16.9 ± 1.6 (12)

Adj. P = 0.57

Adj. P = 0.09

Adj. P = 0.03*

Adj. P = 0.02*

Basal spine density per 100 μm

WT: 21.4 ± 2.1 (7)

WT: 22.1 ± 2.2 (5)

WT: 27.9 ± 3.3 (5)

WT: 20.6 ± 3.1 (8)

SOD1: 16.7 ± 2.9 (5)

SOD1: 16.7 ± 3.4 (5)

SOD1: 20.3 ± 0.8 (5)

SOD1: 13.2 ± 1 (12)

Adj. P = 0.79

Adj. P = 0.66

Adj. P = 0.21

Adj. P = 0.04*

  1. All analyses were unpaired two-way ANOVAs for age and genotype of LVPNs from the somatosensory cortex comparing soma volume (Age: P = 0.0100*; Genotype: P = 0.1295), total dendritic arbor length (Age: P < 0.0001****; Genotype: P = 0.9331), apical dendritic arbor length (Age: P < 0.0001****; Genotype: P = 0.6179), basal dendritic arbor length (Age: P = 0.0014**; Genotype: P = 0.8894), mean basal dendritic tree length (Age: P = 0.0048**; Genotype: P = 0.0773), apical dendritic reach (Age: P < 0.0001****; Genotype: P = 0.2972), basal dendritic reach (Age: P = 0.1037; Genotype: P = 0.2159), apical branch ramifications (Age: P = 0.0011**; Genotype: P = 0.7403), basal branch ramifications (Age: P = 0.7061; Genotype: P = 0.4196), apical dendritic spine density per 100 μm (Age: P = 0.1270; Genotype: P < 0.0001****) and basal dendritic spine density per 100 μm (Age: P = 0.0343*; Genotype: P = 0.0009***). Bonferroni post-tests were used to compare the effect of genotype at each age group, with the adjusted P-value (adj. P) reported in the table for parameters that had significant genotype effects. Each animal used (n) contains mean values from a minimum of 2 individual neurons, specifically P8-15 (WT: 15 neurons; SOD1: 10 neurons), P28-35 (WT: 12 neurons; SOD1: 17 neurons), P65-75 (WT: 14 neurons; SOD1: 12 neurons) and P120 (WT: 20 neurons; SOD1: 28 neurons)
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Acta Neuropathologica Communications

ISSN: 2051-5960

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