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Table 4 Morphometric dendritic and dendritic spine parameters of pyramidal neurons within the MPFC. All data presented as mean ± SEM

From: Marked changes in dendritic structure and spine density precede significant neuronal death in vulnerable cortical pyramidal neuron populations in the SOD1G93A mouse model of amyotrophic lateral sclerosis

Region

P8-15 (n) Neonatal

P28-35 (n) Pre-symptomatic

P65-75 (n) Onset

P120 (n) Mid-disease

Soma volume (μm3)

WT: 951.8 ± 263.4 (7)

WT: 1337.9 ± 150.1 (5)

WT: 1215.4 ± 205.2 (5)

WT: 860.1 ± 112.3 (8)

SOD1: 1337.1 ± 216.9 (5)

SOD1: 1236.3 ± 267.4 (5)

SOD1: 1203.6 ± 266.1 (5)

SOD1: 773.5 ± 132.6 (12)

Not significant

Not significant

Not significant

Not significant

Total arbor length (μm)

WT: 1658 ± 119 (7)

WT: 2263 ± 175 (5)

WT: 2343 ± 190 (5)

WT: 2675 ± 321 (8)

SOD1: 2446 ± 336 (5)

SOD1: 2074 ± 238 (5)

SOD1: 1243 ± 170 (5)

SOD1: 1294 ± 133 (12)

Adj. P = 0.09

Adj. P > 0.99

Adj. P = 0.01*

Adj. P < 0.0001****

Apical arbor length (μm)

WT: 783 ± 145 (7)

WT: 1152 ± 84 (5)

WT: 912 ± 125 (5)

WT: 1378 ± 191 (8)

SOD1: 1081 ± 204 (5)

SOD1: 1137 ± 221 (5)

SOD1: 564 ± 119 (5)

SOD1: 810 ± 197 (12)

Not significant

Not significant

Not significant

Not significant

Basal arbor length (μm)

WT: 915 ± 100 (7)

WT: 1111 ± 175 (5)

WT: 1430 ± 102 (5)

WT: 1296 ± 149 (8)

SOD1: 1365 ± 225 (5)

SOD1: 937 ± 238 (5)

SOD1: 879 ± 88 (5)

SOD1: 484 ± 60 (12)

Adj. P = 0.048*

Adj. P > 0.99

Adj. P = 0.0008***

Adj. P < 0.0001****

Mean basal tree length (μm)

WT: 233 ± 24 (7)

WT: 305 ± 9 (5)

WT: 350 ± 55 (5)

WT: 381 ± 32 (8)

SOD1: 424 ± 93 (5)

SOD1: 273 ± 27 (5)

SOD1: 190 ± 17 (5)

SOD1: 133 ± 15 (12)

Adj. P = 0.004**

Adj. P > 0.99

Adj. P = 0.03*

Adj. P < 0.0001****

Apical reach (μm)

WT: 402 ± 58 (7)

WT: 517 ± 35 (5)

WT: 432 ± 32 (5)

WT: 573 ± 55 (8)

SOD1: 355 ± 21 (5)

SOD1: 460 ± 63 (5)

SOD1: 372 ± 61 (5)

SOD1: 365 ± 32 (12)

Adj. P > 0.99

Adj. P > 0.99

Adj. P > 0.99

Adj. P < 0.002**

Basal reach (μm)

WT: 207 ± 28 (7)

WT: 226 ± 25 (5)

WT: 248 ± 15 (5)

WT: 216 ± 14 (8)

SOD1: 302 ± 56 (5)

SOD1: 192 ± 7 (5)

SOD1: 148 ± 20 (5)

SOD1: 135 ± 23 (12)

Not significant

Not significant

Not significant

Not significant

Apical ramifications

WT: 5.3 ± 0.8 (7)

WT: 5.9 ± 0.6 (5)

WT: 5.8 ± 0.4 (5)

WT: 6.9 ± 0.8 (8)

SOD1: 7.5 ± 0.6 (5)

SOD1: 6.6 ± 0.9 (5)

SOD1: 5.5 ± 1.5 (5)

SOD1: 5.7 ± 0.8 (12)

Not significant

Not significant

Not significant

Not significant

Basal ramifications

WT: 3.2 ± 0.3 (7)

WT: 4.2 ± 0.5 (5)

WT: 4 ± 0.3 (5)

WT: 4.4 ± 0.2 (8)

SOD1: 4.4 ± 0.3 (5)

SOD1: 4.2 ± 0.1 (5)

SOD1: 3.4 ± 0.4 (5)

SOD1: 3.1 ± 0.3 (12)

Not significant

Not significant

Not significant

Not significant

Apical spine density per 100 μm

WT: 21.1 ± 2.7 (7)

WT: 25.3 ± 1.1 (5)

WT: 33.8 ± 1.6 (5)

WT: 27.5 ± 1.6 (8)

SOD1: 21 ± 3.4 (5)

SOD1: 20 ± 2.8 (5)

SOD1: 15.4 ± 2.5 (5)

SOD1: 19.6 ± 2.5 (12)

Adj. P > 0.9999

Adj. P = 0.8341

Adj. P = 0.0002***

Adj. P = 0.0415*

Basal spine density per 100 μm

WT: 15.9 ± 1.7 (7)

WT: 22.4 ± 2.2 (5)

WT: 26.2 ± 1.6 (5)

WT: 22.1 ± 1.8 (8)

SOD1: 18.4 ± 1.2 (5)

SOD1: 12.5 ± 1.4 (5)

SOD1: 14.3 ± 2.5 (5)

SOD1: 14.2 ± 1.6 (12)

Adj. P > 0.99

Adj. P = 0.01*

Adj. P = 0.002**

Adj. P = 0.004**

  1. All analyses were unpaired two-way ANOVAs for age and genotype of pyramidal neurons from the MPFC comparing soma volume (Age: P = 0.0637; Genotype: P = 0.7515), total dendritic arbor length (Age: P = 0.5093; Genotype: P = 0.0063**), apical dendritic arbor length (Age: P = 0.0763; Genotype: P = 0.1713), basal dendritic arbor length (Age: P = 0.1392; Genotype: P = 0.0005***), mean basal dendritic tree length (Age: P = 0.2178; Genotype: P = 0.0238*), apical dendritic reach (Age: P = 0.1008; Genotype: P = 0.0122*), basal dendritic reach (Age: P = 0.0267*; Genotype: P = 0.1295), apical branch ramifications (Age: P = 0.8608; Genotype: P = 0.5692), basal branch ramifications (Age: P = 0.4943; Genotype: P = 0.4028), apical dendritic spine density per 100 μm (Age: P = 0.6262; Genotype: P = 0.0001***) and basal dendritic spine density per 100 μm (Age: P = 0.4772; Genotype: P < 0.0001****). Bonferroni post-tests were used to compare the effect of genotype at each age group, with the adjusted P-value (adj. P) reported in the table for parameters that had significant genotype effects. Each animal used (n) contains mean values from multiple individual neurons, specifically P8-15 (WT: 12 neurons; SOD1: 10 neurons), P28-35 (WT: 10 neurons; SOD1: 10 neurons), P65-75 (WT: 10 neurons; SOD1: 13 neurons) and P120 (WT: 16 neurons; SOD1: 20 neurons)