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Table 3 Morphometric dendritic and dendritic spine parameters of LII-IIIPNs within the motor cortex. All data presented as mean ± SEM

From: Marked changes in dendritic structure and spine density precede significant neuronal death in vulnerable cortical pyramidal neuron populations in the SOD1G93A mouse model of amyotrophic lateral sclerosis

Region

P8-15 (n) Neonatal

P28-35 (n) Pre-symptomatic

P65-75 (n) Onset

P120 (n) Mid-disease

Soma volume (μm3)

WT: 587.5 ± 98.1 (7)

WT: 1338.5 ± 119.3 (5)

WT: 1297.5 ± 194.5 (5)

WT: 998.3 ± 112.4 (8)

SOD1: 997.0 ± 266.0 (5)

SOD1: 1063.1 ± 271.3 (5)

SOD1: 1218.4 ± 356.2 (5)

SOD1: 701.9 ± 101.4 (12)

Not significant

Not significant

Not significant

Not significant

Total arbor length (μm)

WT: 2005 ± 414 (7)

WT: 2626 ± 101 (5)

WT: 2468 ± 208 (5)

WT: 2747 ± 288 (8)

SOD1: 1709 ± 244 (5)

SOD1: 2116 ± 241 (5)

SOD1: 1831 ± 338 (5)

SOD1: 1883 ± 196 (12)

Adj. P > 0.99

Adj. P > 0.99

Adj. P = 0.67

Adj. P < 0.047*

Apical arbor length (μm)

WT: 762 ± 159 (7)

WT: 1452 ± 165 (5)

WT: 1052 ± 141 (5)

WT: 1602 ± 176 (8)

SOD1: 922 ± 47 (5)

SOD1: 899 ± 77 (5)

SOD1: 785 ± 176 (5)

SOD1: 947 ± 107 (12)

Adj. P > 0.99

Adj. P = 0.09

Adj. P > 0.99

Adj. P < 0.001**

Basal arbor length (μm)

WT: 1242 ± 268 (7)

WT: 1174 ± 167 (5)

WT: 1415 ± 77 (5)

WT: 1094 ± 174 (8)

SOD1: 787 ± 219 (5)

SOD1: 1208 ± 202 (5)

SOD1: 1046 ± 164 (5)

SOD1: 963 ± 104 (12)

Not significant

Not significant

Not significant

Not significant

Mean basal tree length (μm)

WT: 300 ± 57 (7)

WT: 290 ± 43 (5)

WT: 314 ± 24 (5)

WT: 320 ± 47 (8)

SOD1: 225 ± 58 (5)

SOD1: 317 ± 44 (5)

SOD1: 283 ± 27 (5)

SOD1: 257 ± 27 (12)

Not significant

Not significant

Not significant

Not significant

Apical reach (μm)

WT: 303 ± 28 (7)

WT: 599 ± 48 (5)

WT: 482 ± 65 (5)

WT: 450 ± 48 (8)

SOD1: 424 ± 74 (5)

SOD1: 443 ± 29 (5)

SOD1: 398 ± 64 (5)

SOD1: 321 ± 37 (12)

Not significant

Not significant

Not significant

Not significant

Basal reach (μm)

WT: 199 ± 22 (7)

WT: 209 ± 21 (5)

WT: 249 ± 18 (5)

WT: 205 ± 18 (8)

SOD1: 178 ± 34 (5)

SOD1: 229 ± 29 (5)

SOD1: 193 ± 26 (5)

SOD1: 188 ± 26 (12)

Not significant

Not significant

Not significant

Not significant

Apical ramifications

WT: 5.2 ± 0.7 (7)

WT: 5.7 ± 0.6 (5)

WT: 5.9 ± 0.3 (5)

WT: 7.9 ± 0.7 (8)

SOD1: 8.5 ± 0.8 (5)

SOD1: 5.3 ± 0.3 (5)

SOD1: 5.7 ± 1 (5)

SOD1: 6.3 ± 0.4 (12)

Not significant

Not significant

Not significant

Not significant

Basal ramifications

WT: 3.9 ± 0.5 (7)

WT: 3.7 ± 0.2 (5)

WT: 3.9 ± 0.2 (5)

WT: 4.3 ± 0.3 (8)

SOD1: 3.5 ± 0.3 (5)

SOD1: 4.8 ± 0.4 (5)

SOD1: 3.4 ± 0.3 (5)

SOD1: 3.9 ± 0.2 (12)

Not significant

Not significant

Not significant

Not significant

Apical spine density per 100 μm

WT: 29.8 ± 1.6 (7)

WT: 34.2 ± 4.4 (5)

WT: 36.3 ± 2 (5)

WT: 22.9 ± 2.3 (8)

SOD1: 19.8 ± 3 (5)

SOD1: 19.7 ± 2.2 (5)

SOD1: 25.7 ± 3.1 (5)

SOD1: 22.6 ± 2 (12)

Adj. P = 0.0277*

Adj. P = 0.0039**

Adj. P = 0.0494*

Adj. P > 0.9999

Basal spine density per 100 μm

WT: 17.4 ± 1.8 (7)

WT: 26.7 ± 3.2 (5)

WT: 27.5 ± 3.5 (5)

WT: 17.2 ± 2.5 (8)

SOD1: 20.9 ± 4.3 (5)

SOD1: 16.5 ± 2.1 (5)

SOD1: 19.6 ± 1.7 (5)

SOD1: 18.3 ± 1.9 (12)

Not significant

Not significant

Not significant

Not significant

  1. All analyses were unpaired two-way ANOVAs for age and genotype of LII-IIIPNs from the motor cortex comparing soma volume (Age: P = 0.0272*; Genotype: P = 0.6421), total dendritic arbor length (Age: P = 0.3006; Genotype: P = 0.0082**), apical dendritic arbor length (Age: P = 0.0185*; Genotype: P = 0.0038**), basal dendritic arbor length (Age: P = 0.5556; Genotype: P = 0.0889), mean basal dendritic tree length (Age: P = 0.8449; Genotype: P = 0.2142), apical dendritic reach (Age: P = 0.0218*; Genotype: P = 0.0963), basal dendritic reach (Age: P = 0.5799; Genotype: P = 0.3441), apical branch ramifications (Age: P = 0.0311*; Genotype: P = 0.1325), basal branch ramifications (Age: P = 0.2204; Genotype: P = 0.8882), apical dendritic spine density per 100 μm (Age: P = 0.0155*; Genotype: P < 0.0001****) and basal dendritic spine density per 100 μm (Age: P = 0.1380; Genotype: P = 0.0877). Bonferroni post-tests were used to compare the effect of genotype at each age group, with the adjusted P-value (adj. P) reported in the table for parameters that had significant genotype effects. Each animal used (n) contains mean values from a minimum of 2 individual neurons, specifically P8-15 (WT: 20 neurons; SOD1: 10 neurons), P28-35 (WT: 11 neurons; SOD1: 11 neurons), P65-75 (WT: 11 neurons; SOD1: 13 neurons) and P120 (WT: 21 neurons; SOD1: 25 neurons)