Fig. 6

Decreased apical and basal dendritic spine density of SOD1 pyramidal neurons within the MPFC commenced from P28-35, compared to WT controls. High-magnification images of apical dendrites from P8-15, P28-35, P65-75 and P120 WT (a, c, e, g) and SOD1 (b, d, f, h) MPFC are shown. High-magnification images of basal dendrites from P8-15, P28-35, P65-75 and P120 WT (i, k, m, o) and SOD1 (j, l, n, p) motor cortex are also shown. Examples of apical and basal dendritic spines are identified with red arrowheads. q is a scatterplot quantifying significantly decreased apical dendritic spine density per 100 μm of SOD1 LVPNs (grey squared) compared to WT controls (white circles) at P65-75 and P120. r is a scatterplot quantifying significantly decreased basal dendritic spine density per 100 μm of SOD1 LVPNs (grey squared) compared to WT controls (white circles) at P28-35, P65-75 and P120. All data mean ± SEM, with two-way ANOVAs followed by Bonferroni post-tests, *P < 0.05 and **P < 0.01. n = 7, 5, 5 and 8 for WT P8-15, P28-35, P65-75 and P120 respectively. n = 6, 5, 5 and 12 for SOD1 P8-15, P28-35, P65-75 and P120 respectively. Scale bar: h and p, 5 μm