Fig. 2

Histological evidence of rotenone-induced neurodegeneration in the SN, striatum and retina. a Quantitative grading of TH immunostaining on coronal sections of the SN reveals that significant loss of dopaminergic neurons is seen at day 60 of the rotenone treatment, yet not at day 10 or 20. b Significantly reduced immunolabeling is also seen in the striatum of rotenone-treated rats at day 60. c Significant increase in Alpha Syinuclein staining was also present in the retina in rotenone treated rats at 60 days. d Representative images of TH immunostainings in the SN and striatum, revealing dopaminergic cell loss after 60 days of rotenone treatment alongside retinal histological section revealing increase in alpha synuclein staining in rotenone treated animals. Scale bar, 500 μm