Fig. 4

Immune response and blood brain barrier permeability are not altered in microglial LRP1 deficient mice. Seven days after MOG immunization, draining lymph nodes were isolated from Cx3cr1 ^creER-Lrp1 ^fl/fl and Lrp1 ^fl/fl mice and incubated with MOG. a-b IFN-γ and IL-17A production were determined by ELISA after 24 h. c BrdU incorporation by T cells was determined after 16 h by flow cytometry (n = 3–5 mice were used per group per experiment). Statistical analysis was performed with 2-way ANOVA. d The amount of fluorescein present in brains following LPS treatment (6 mg/kg) was measured in Cx3cr1 ^creER-Lrp1 ^fl/fl and Lrp1 ^fl/fl mice to assess function of the blood brain barrier. Statistical analysis was performed with Student’s t-Test; means ± SEM