Fig. 3

Microglial LRP1 deficient mice have increased demyelination and numbers of immune cells in the CNS during EAE. Spinal cord sections of Cx3cr1 ^creER-Lrp1 ^fl/fl and Lrp1 ^fl/fl mice were stained with Luxol Fast Blue a at day 30 (n = 4 for each group), and CD3 b (n = 4 for each group) and Iba1 c (n = 4 for each group) at day 15. Cells were manually quantified. For LFB, 2 sections per spinal cord level are plotted from each animal. For CD3 and Iba1, each point represents one animal. Immune cells were isolated by percoll gradient from spinal cords of Cx3cr1 ^creER-Lrp1 ^fl/fl and Lrp1 ^fl/fl mice. Number of d peripheral myeloid cells (CD11b^hiCD45^hi), e T cells (TCRβ+) cells, f microglia (CD11b^hiCD45^mid), and g B cells (CD19+) were determined by flow cytometry analysis (n = 3 for each group). *p < 0.05, **p < 0.01; Student’s t-test, mean ± s.e.m