Fig. 4
Astrocyte reactivity is elevated but microgliosis is dampened in APPSWE/PS1ΔE9 x IFNAR1−/− mice. a Representative cortical sections from 9 month old APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− mice stained with anti-GFAP using fluorescence immunohistochemistry. b Integrated density values of positive GFAP immunofluorescence were calculated from entire cortical regions of APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− mice (3 sections per mouse). c High power magnification images of APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− mouse cortical sections triple-labelled with DAPI, anti-GFAP and anti-WO-2. d Representative cortical sections from 9 month old APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− mice stained with anti-IBA-1 using fluorescence immunohistochemistry. e Integrated density values of positive IBA-1 immunofluorescence were calculated from entire cortical regions of APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− mice (3 sections per mouse). f High power magnification images of APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− mouse cortical sections triple-labelled with DAPI, anti-IBA-1 and anti-WO-2. b Scale bars: low power = 200 μm; high power = 30 μm. All data is displayed as box plots described in the statistical analysis section in Materials and Methods (n = 9 per genotype; •represents outlier value; **p < 0.01, ***p < 0.001). See Additional file 2: Table S1 for further analysis