Fig. 3
The type-1 IFN and pro-inflammatory cytokine response is attenuated upon removal of IFNAR1 in APPSWE/PS1ΔE9 mice. a Q-PCR of cortical tissue isolated from 9 month old wildtype, IFNAR1−/−, APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− littermate controls analyzing IFNα, IRF7, IRF3 and IRF8 transcript levels. b Representative immunoblot of Tris–HCl soluble cortical protein lysates isolated from 9 month old wildtype, IFNAR1−/−, APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− mice using anti-p-Stat-3. c Densitometry of cortical p-Stat-3 levels in APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− mice is shown. d Q-PCR of cortical tissue isolated from 9 month old wildtype, IFNAR1−/−, APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− littermate controls analyzing IL-1β, IL-6 and TNFα transcript levels. For Q-PCR, all samples were normalized back to the Ct value of the housekeeping gene GAPDH (ΔCt). The mRNA of the variant genotype groups were then expressed relative to their gene-specific wildtype littermate controls (fold change, ΔΔCt). For densitometry, total Stat-3 levels were normalized to the β-actin loading control and p-Stat-3 intensity was calculated relative to this value (p-Stat-3/(Stat-3/β-actin). Intensity values of the APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− mouse groups are expressed as fold change relative to wildtype littermate control levels (represented by the dashed line). Immunodetection of β-actin was used to ascertain loading quantities. Data are displayed as box plots box plots described in the statistical analysis section in Materials and Methods (Q-PCR: n = 9 per genotype; Western blotting: n = 4 per genotype; •represents outlier value; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). See Additional file 2: Table S1 for further analysis