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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Deletion of the type-1 interferon receptor in APPSWE/PS1ΔE9 mice preserves cognitive function and alters glial phenotype

Fig. 1

Removal of IFNAR1 in APPSWE/PS1ΔE9 mice confers modest reductions in Aβ monomer levels but not plaque burden. Representative cortical sections from 9 month old a APPSWE/PS1ΔE9 and b APPSWE/PS1ΔE9 x IFNAR1−/− mice stained with anti-Aβ mAb WO-2 using fluorescence immunohistochemistry (scale bar = 200 μm). c Aβ plaques were counted from entire cortical regions of APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− mice (3 sections per mouse, •represents outlier value). d Cortical plaque burden was calculated by quantifying Aβ plaque immunofluorescence relative to total cortical area from these same cortical slices of APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− mice. e PBS-T-soluble and f PBS-T-insoluble Aβ1:40 levels in APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− mouse cortical lysates were quantified by ELISA. g Representative immunoblot of Tris–HCl soluble cortical protein lysates isolated from 9 month old wildtype, IFNAR1−/−, APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− mice using the anti-Aβ mAb WO-2. Multiple amyloid species can be detected including endogenous APP-CTF (muAPP-CTF), transgenic APP-CTF (TgAPP-CTF), Aβ trimers (3-mer) and Aβ monomers. A long exposure (LE) was used to enhance detection of Aβ monomer levels. Densitometry of h Transgenic APP-CTF, i endogenous murine APP-CTF, j Aβ monomer and k 3-mer levels in APPSWE/PS1ΔE9 and APPSWE/PS1ΔE9 x IFNAR1−/− mice is shown. All densitometry is expressed as a ratio of Aβ monomer:β-actin or Aβ trimer:β-actin raw pixel intensities. Immuno-detection of β-actin was used to ascertain loading quantities. Data is presented as box plots described in the statistical analysis section in Materials and Methods (immunohistochemistry: n = 9 per genotype; ELISA and Western blotting: n = 6 (APPSWE/PS1ΔE9), n = 4 (APPSWE/PS1ΔE9 x IFNAR1−/−); *p < 0.05, ****p < 0.0001). See Additional file 2: Table S1 for further analysis

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