Fig. 2
From: Modeling Alexander disease with patient iPSCs reveals cellular and molecular pathology of astrocytes
iPSCs from AxD patients and healthy controls could differentiate into astrocytes with high purity. a Schematic procedures for astroglial differentiation. b Differentiated iPSCs at day 24 expressed neural stem cell markers NESTIN and GFAP. Neural cells at day 60 expressed neuronal or astrocytic marker TUJ1 or GFAP. Most enriched astrocytes expressed GFAP. Scale bars = 20 μm. c Estimation of astroglial differentiation from control and AxD iPSCs. After 180 days of differentiation, astrocytes were immunostained with an antibody against S100β (red color). Scale bars = 20 μm. d Calculated purity of astrocytic differentiation Data represent mean ± SD (biological replicates, n = 3 from randomly picked fields per clone). Two-way analysis of variance (ANOVA) did not show significant variation. F (5, 12) =0.2432; p = 0.935