Fig. 6

T5x microglia phagocytose more Aβ than their 5xfAD counterparts. (a) Equivalent 7 month old T5x and 5xfAD sections were labeled for microglia (IBA1, green), Aβ (82E1, red), and microglial phagolysosomes (CD68, blue). IMARIS bitplane software rendering of confocal Z-stacks was then used to calculate the volume of phagolysosomes within microglia and then the volume of internalized Aβ within those CD68⁺ microglial phagolysosomses, depicted in greyscale in A. Together, this analysis demonstrate that although T5x and 5xfAD microglia exhibit no differences in phagolysosome volume per microglia (b), the amount of Aβ within the phagolysosomes of T5x microglia was significantly increased (c) versus 5xfAD microglia. Data are represented as mean ± SEM, normalized to % of 5xfAD group, n ≥ 9 mice/group. * Indicates p < 0.05 for both ANOVA and Fisher’s protected least-significant difference (PLSD) post hoc tests. Scale Bar = 20 μm