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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Increased tauopathy drives microglia-mediated clearance of beta-amyloid

Fig. 5

T5x mice exhibit decreased amyloid burden compared to 5xfAD littermates. a Amyloid deposition was quantified using immunofluorescent labeling of Aβ (82E1) followed by confocal Z-stack imaging and IMARIS bitplane software analysis within CA1 of the hippocampus (CA1) the dentate gyrus (DG), retrosplenial cortex (RSC), and parietal association cortex (PAC). b Amyloid plaque volume was significantly decreased in T5x mice relative to 5xfAD littermates in CA1 (p = 0.03), RSC (p = 0.04), and the PAC (0.03), whereas a nonsignificant reduction was observed in the dentate gyrus (p = 0.07). c To determine whether the observed reduction in Aβ pathology resulted from a change in APP transgene expression, human full-length APP (6E10; ~100 kDA) and C99 (6E10; ~13 kDA) was examined and quantified by western blot, revealing no differences between T5x and 5xfAD groups (d, e). Next, levels of soluble and insoluble Aβ38, 40 and 42 were examined using a Mesoscale Devices (MSD) multiplex ELISA. Interestingly, while soluble levels of Aβ where unchanged between T5x and 5xfAD groups (f), levels of insoluble Aβ38, 40 and 42 were significantly decreased in T5x mice relative to 5xfAD littermates (g). Data are represented as mean ± SEM, n ≥ 9 mice/group. * indicates p < 0.05 for both ANOVA and Fisher’s protected least-significant difference (PLSD) post hoc tests between T5x and 5x groups for that brain region or Aβ species. Scale Bar = 50 μm

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