Fig. 3

The anatomical localization of pTDP-43 deposits forming granular and dot-like DNs. (a-l) Double-labeling immunofluorescence in the temporal cortex (a-f) and globus pallidus (g-l) from two different cases of Type 2b. Phosphorylated TDP-43 (pTDP-43)-positive granular and dot-like structures are observed along SMI-32-positive dendrites (a, g: green, pTDP-43; b, h: red, SMI-32; c, i: merged). No co-localization of pTDP-43 and synaptophysin is evident (d, j: green, pTDP-43; e, k: red: synaptophysin; f, l: merged). Close association with pTDP-43-positive structures and synaptophysin-positive structures is observed in the globus pallidus (j-l). There is evidence of contact between pTDP-43- and synaptophysin-positive structures, suggesting that pTDP-43 is localized in the dendritic spines (l, insert). Scale bars: a-f = 5 μm; g-l = 10 μm