Fig. 1
From: Uncoupling neuronal death and dysfunction in Drosophila models of neurodegenerative disease
Age-dependent changes in retinal function monitored using electroretinograms. a Representative ERG traces are shown for each genotype examined at 1, 10, and 30 days. The overall depolarization amplitude (Δ) reflects the output of the phototransduction cascade. Synaptic neurotransmission generates the ON and OFF transient potentials (arrowheads). b Quantification of ERG depolarization amplitude by genotype and age (2-way ANOVA model F-test, p < 0.001). Rh1 > Tau causes a rapid and progressive loss of ERG depolarization whereas Rh1 > αSyn causes a relatively modest and late decline. c & d Quantitation of the synaptic ON and OFF transient potentials reveals efficacy of photoreceptor neurotransmission (2-way ANOVA p < 0.001 for each model). The measurement of the ON transient c is less reliable due to its small magnitude in controls (panel A). Rh1 > Tau and Rh1 > αSyn show progressive loss in OFF transients d. ERGs were recorded from at least 6 animals per genotype (genotype, n1, n10, n30): Rh1-Gal4/+, 17, 13, 14; Rh1 > Tau, 16, 16, 11; Rh1 > Aß, 6, 12, 6; Rh1 > αSyn 9, 10, 14. Subsetted t-tests were performed for post-hoc comparisons of control animals with each experimental genotype, considering each timepoint independently, and p-values were adjusted using the Bonferroni method. *, p < 0.05; **, p < 0.01; ***p < 0.001